WebMar 8, 2024 · Abstract. This protocol details the steps required to wash a MInION flowcell using Oxford Nanopore's Flowcell Wash kit (EXP-WSH003). The kit contains a DNase I … WebFeb 3, 2024 · Five of these R9 ONT flow cells were washed and reused according to the Flow Cell Wash kit and protocol (EXP-WSH003, version: WFC_9088_v1_revB_18Sept2024). Sequencing was performed using default settings on an ONT (Oxford, UK) MinION (MinIon 19.12.5 or 19.10.1) using MinKNOW default settings …
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WebMar 17, 2024 · Prior to reuse, the flow cells were purged according to the manufacturer’s protocol using flow cell wash kits (EXP-WSH003) (ONT). 2.5 Sequencing data analysis. Sanger sequencing data was analyzed using SEQUENCE Pilot Software [v 3.4.2] (JSI medical systems GmbH, Ettenheim, Germany). WebAfter 24 h, a nuclease-digest was performed utilizing the EXP-WSH003 (ONT) flow cell wash kit and sequencing was continued with a new generated library. Raw reads were basecalled with GUPPY v.3.2.6 GPU version. Genomes were assembled using CAN U1, 2 v.1.9. assuming a genome size of 40 Mbp grassland major types of flora
Flow Cell Wash Kit Exp wsh004 WFC - 9120 - v1 - Revb
WebNov 29, 2024 · Flow Cell Wash Kit (o ptional) ONT. EXP-WSH003. Flow Cell Priming K it. ONT. EXP-FLP002. Component. Supplier. Part Number. ... 15 Open the MinION dev ice lid and slide the flow cell under the clip. Analysis of sequence information on any Oxford Nanopore platform occurs in real-time, allowing users to run a flow cell until sufficient data has been collected to fulfil the experimental requirements, at which point the run can be stopped. The Flow Cell Wash Kit provides a highly effective means of removing a … See more In sequencing experiments where an accumulation of pores in the “recovering” state (“unavailable” in the detailed view) is observed, the rate of data acquisition drops as fewer pores are available to accept and … See more Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box. Products are shipped to customers within the USA and EU Monday to … See more WebOct 10, 2024 · Each flow cell was reused up to 4 times after washing with the Flow Cell Wash Kit (EXP-WSH003, Oxford Nanopore Technologies). When multiplexing retrospective samples, up to 10 libraries were pooled and sequenced for up to 24 h. Nanopore Data Analysis Pipeline. grassland meadow